NewsWise – Apple fruit quality depends a lot on symptoms such as firmness, taste and color, which are regulated by complex genetic networks. Among them, perseverance is particularly important because it affects storage, transportability and marketing ability. The previous attempts to map a strong -related genes faced challenges due to Apple’s long adolescent period and complex genome. Although many QIL and candidates like genes Mdacs1 And Mderf3 Identified, specific genetic factors that run natural variations in firmness are unclear. Based on these challenges, apple fruit is a strong requirement to study high-resolution genetic mapping and functional verification to dissect the molecular basis of firmness and ripening.
A research team from Northwest A&F University in collaboration with Yale University, A published A Study (Doi: 10.1093/HR/UHAE284) In October 8, 2024, Horticulture researchThe team rejected the genome of 294 apple hybrids obtained from ‘Fuji’ and ‘Cripps Pink’ and identified an important SNP mutation. Mdnac5 The gene that affects the firmness and ripening of the apple. Mixing high density genetic mapping with functional assays, the study provides a wide molecular structure Mdnac5 Diversity for ethylene biosynthesis and fruit texture, providing valuable equipment for apple breeding programs.
In this study, researchers built a high -density genetic map using 5014 bin markers covering 17 apple chromosomes. He identified 60 QTL associated with fruit -related symptoms, of which a major place stood out on chromosome 3. Within the region, he pointed to a fruitless A-to-T SNP Mdnac5 It replaces encoded amino acids from Methionin to Lucene. Functional analysis showed that Mdnac5a The version (an allele) accelerates cooking and reduces the firmness of the fruits, while Mdnac5t The variant delays ripening and maintains firmness. Overseas studies in apple Callie and Tomato fruit showed that Mdnac5a Leaded to high production of Methionin and ACC, compared to the leading forearm in ethylene biosynthesis, Mdnac5tFurther experiments showed that both variants tie promoters directly Mdacs1 And Mderf3Two ethylene related genes, but Mdnac5a Performed strong regulatory activity. By this double regulation Mdnac5 And conversation with it Mderf3 Modify ethylene levels, eventually control the firmness and ripening of fruits. These findings throw light on the genetic system of fine tuned behind the symptoms of the quality of apple fruit.
“Identify Mdnac5 As a major genetic regulator of apple persistence, fruit crop is a milestone in genomics, “Dr. Yazo Yang said, one of the concerned writers of the study.” Our findings not only deepen the understanding of control of ripening control in climacteric fruits, but also provide a direct molecular goal for Apple reproductive programs to improve storage and consumer properties. Manipulated Mdnac5 Alils, we can expand the shelf life without compromising the taste, to meet the apple’s specific market demands. ,
This discovery opens exciting possibilities for accurate breeding in apples. By selecting specific Mdnac5 Variants, Breeders can cultivate over the level of sequential firms and time of ripening, adaptation of apples for prolonged storage, better transport flexibility and enhanced consumer appeal. Additionally, the study throws light on how genetic mapping and functional verification on fine scale can accelerate the growth of better fruit crops. Beyond apple, similar strategies can be applied to other climbers fruits where texture and ripening are the major commercial symptoms, eventually contributing to reducing food loss and increasing agricultural stability.
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Reference
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Original source url
https://doi.org/10.1093/hr/uhae284
Money information
This work has been made by the Vayon Experimental Displaying Station (2024WNXNZX-1) and Chinese PostDoATARAL SINS Foundation (2024 M75263636363636363636363636363636363636363636363636363636363636363636 Was done for
About this Horticulture research
Horticulture research Nanjing is an open access magazine of the Agricultural University and is at number one in the horticulture category of Journal Catation Report ⢠from Clearivate, 2023. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters. Interaction of inter-species, physiology, and the origin and domination of crops.
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